ISOprint - Analytical tools for the determination of isotopi
ISOprint - Analytical tools for the determination of isotopi
Disciplines
Biology (10%); Chemistry (70%); Agriculture and Forestry, Fishery (20%)
Keywords
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DGT,
Isotopes,
Soil,
Provenance
ISOprint - Development of Diffusive Gradient in Thin Films (DGT) multi-collector ICP-MS techniques for location-specific isotopic fingerprinting of S, Sr and Pb in soils as a tool for the provenance determination of primary agricultural products The aim of this project is to develop a technique for sampling the biologically available (bioavailable) fraction of sulfur (S), strontium (Sr) and lead (Pb) from soils. Subsequently, the isotopic composition of these elements will analyzed. The motivation behind is that a site-specific variation of isotopic composition of S, Sr and Pb in soil can act as specific fingerprint. This fingerprint is mirrored in plants growing on this soil. The isotopic difference can be caused by variations in the geology, by natural processes (e.g. microbial activities) or by anthropogenic activities (e.g. by combustion of fossil fuels containing S or petrol containing Pb). Diffusive gradients in thin films (DGT) represent an efficient passive sampling technique. DGT takes up elements from soil in the same way plants do, simply by diffusion. In this (bioavailable) fraction, the isotopic composition of the investigated elements will be analyzed by a dedicated mass spectrometer. Within this project, we want to develop a novel technique, which enables to take up S, Sr and Pb from soil without causing any change in the isotopic composition of these elements. takes up the part of S, Sr and Pb, which would be taken up by plants (bioavailable fraction) and the isotopic composition of these elements taken up by DGT from soil equals to that of plants grown on the same soil. allows for the establishment of a direct link of the chemical information stored in soils to the information found in primary agricultural products and therefore allows for provenancing. For the first time, the bioavailable chemical isotopic information is assessed directly. Within this project, new binding layers for S and Sr will be developed making use of the expertise of the collaborating company, which provides an expertise on novel resin materials. The results of this findings will be compared to greenhouse and field experiments. The goal of this approach is that we will be able to determine the site-specific isotopic signal and use it to investigate the origin of agricultural products by comparing their isotopic composition with the bioavailable fraction found in soil.
The analytical method developments accomplished within the ISOprint project enabled the targeted analysis of the elemental content and isotopic composition of sulfur (S), strontium (Sr), and lead (Pb) in biologically available (bioavailable) fractions of soils. Variations in the isotopic composition of these elements in bioavailable soil fractions can act as a site-specific isotopic fingerprint (signature) which is mirrored in plants grown on this soil. Therefore, bioavailable S, Sr, and Pb isotopic signatures are successfully applied as tracers in studies on food provenance and authenticity. However, so far, the bioavailable fractions of elements in soil were usually determined using chemical extractions, which do not mimic element uptake by plants and produce sample matrices that hamper isotope ratio analysis by mass spectrometry. Targeted sampling using the diffusive gradients in thin films (DGT) technique can overcome these issues, as it provides a mechanistic surrogate for elemental bioavailability and allows for matrix separation and analyte preconcentration already during sampling. In this project, new DGT techniques capable of quantitative S, Sr, and Pb sampling from soil were developed and fully validated. The techniques did not cause any significant change in the isotopic composition of these elements, enabling the accurate assessment of the site-specific bioavailable isotopic information. Moreover, the DGT technique was successfully extended for the simultaneous assessment of Sr and Pb concentrations and isotope ratios from the same sample within a single sampling step, which has not been possible before. The comprehensive assessment of the isotopic composition of bioavailable S, Sr, and Pb in a range of soils demonstrated the unique capability of the developed methods for robust soil discrimination according to the geographic location. Greenhouse and field experiments showed that the site- specific isotopic signature of Sr and Pb assessed by DGT from soil equals to that of plants grown on the same soil, confirming the applicability of the method for food provenancing. In addition, several project-related collaborations in the field of DGT-based chemical imaging were accomplished, providing a scientific basis to visualize and quantify bioavailable isotope signatures at high spatial resolution. The innovative methods gained in this project are versatile tools for environmental analysis and source tracing of S, Sr, and Pb, which open new analytical capabilities for the provenance determination of primary agricultural products and can be further extended to other disciplines such as archeology, (food) forensics, (eco)geochemistry, and environmental science.
- Montanuniversität Leoben - 100%
- Sean Mason, University of Adelaide - Australia
- Steffen Happel, TRISKEM International - France
Research Output
- 53 Citations
- 13 Publications
- 2 Methods & Materials
- 3 Disseminations
- 14 Scientific Awards
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2022
Title Selective Diffusive Gradients in Thin Films (DGT) for the Simultaneous Assessment of Labile Sr and Pb Concentrations and Isotope Ratios in Soils DOI 10.1021/acs.analchem.2c00546 Type Journal Article Author Wagner S Journal Analytical Chemistry Pages 6338-6346 Link Publication -
2023
Title Mapping Phosphorus Availability in Soil at a Large Scale and High Resolution Using Novel Diffusive Gradients in Thin Films Designed for X-ray Fluorescence Microscopy DOI 10.1021/acs.est.3c06237 Type Journal Article Author Moens C Journal Environmental Science & Technology Pages 440-448 Link Publication -
2020
Title Two-Dimensional Visualization and Quantification of Labile, Inorganic Plant Nutrients and Contaminants in Soil. DOI 10.3791/61661 Type Journal Article Author Wagner S Journal Journal of visualized experiments : JoVE -
2020
Title Arsenic redox transformations and cycling in the rhizosphere of Pteris vittata and Pteris quadriaurita DOI 10.1016/j.envexpbot.2020.104122 Type Journal Article Author Wagner S Journal Environmental and Experimental Botany Pages 104122 Link Publication -
2020
Title Sub-millimeter distribution of labile trace element fluxes in the rhizosphere explains differential effects of soil liming on cadmium and zinc uptake in maize DOI 10.1016/j.scitotenv.2020.140311 Type Journal Article Author Smolders E Journal Science of The Total Environment Pages 140311 Link Publication -
2022
Title Tandem Probe Analysis Mode for Synchrotron XFM: Doubling Throughput Capacity DOI 10.1021/acs.analchem.1c04255 Type Journal Article Author Doolette C Journal Analytical Chemistry Pages 4584-4593 Link Publication -
2022
Title In situ spatiotemporal solute imaging of metal corrosion on the example of magnesium DOI 10.1016/j.aca.2022.339910 Type Journal Article Author Wagner S Journal Analytica Chimica Acta Pages 339910 Link Publication -
2020
Title Visualising sub-millimetre nutrient distribution in the rhizosphere for understanding root nutrient acquisition processes Type Other Author Santner J -
2020
Title Visualising sub-millimetre nutrient distribution in the rhizosphere for understanding root nutrient acquisition processes Type Other Author Santner J -
2024
Title Compost amendment in urban gardens: elemental and isotopic analysis of soils and vegetable tissues DOI 10.1007/s11356-024-34240-7 Type Journal Article Author Trimmel S Journal Environmental Science and Pollution Research Pages 47022-47038 Link Publication -
2024
Title Green and blue infrastructure as model system for emissions of technology-critical elements DOI 10.1016/j.scitotenv.2024.173364 Type Journal Article Author Trimmel S Journal Science of The Total Environment Pages 173364 Link Publication -
2022
Title Development and application of diffusive gradients in thin films (DGT) techniques for multi-elemental chemical imaging and isotope ratio analysis Type Other Author Wagner S -
2022
Title Development and application of diffusive gradients in thin films (DGT) techniques for multi-elemental chemical imaging and isotope ratio analysis Type Other Author Wagner S
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2016
Link
Title Sulfate-DGT DOI 10.1007/s00216-016-9949-2 Type Technology assay or reagent Public Access Link Link -
2022
Link
Title Strontium-Lead-DGT DOI 10.1021/acs.analchem.2c00546 Type Technology assay or reagent Public Access Link Link
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2022
Title Invited lecture TPr (1) Type Personally asked as a key note speaker to a conference Level of Recognition Continental/International -
2022
Title Invited lecture JIr (1) Type Personally asked as a key note speaker to a conference Level of Recognition Continental/International -
2021
Title Invited lecture SWa (1) Type Personally asked as a key note speaker to a conference Level of Recognition Continental/International -
2021
Title Kardinal Innitzer Förderpreis Type Research prize Level of Recognition National (any country) -
2021
Title SAS Atomic Section Student Award Type Research prize Level of Recognition Continental/International -
2020
Title Invited lecture TPr (2) Type Personally asked as a key note speaker to a conference Level of Recognition Continental/International -
2020
Title Invited lecture SWa (2) Type Personally asked as a key note speaker to a conference Level of Recognition Continental/International -
2019
Title Poster Prize European Winter Conference on Plasma Spectrochemistry Type Poster/abstract prize Level of Recognition Continental/International -
2019
Title Best Talk Award ASAC Junganalytiker*innen Forum Type Research prize Level of Recognition National (any country) -
2019
Title Invited lecture JIr (2) Type Personally asked as a key note speaker to a conference Level of Recognition Continental/International -
2019
Title Invited lecture TPr (3) Type Personally asked as a key note speaker to a conference Level of Recognition Continental/International -
2019
Title Invited lecture JIr (3) Type Personally asked as a key note speaker to a conference Level of Recognition Continental/International -
2018
Title Best Talk Award ICP-MS User Meeting Type Research prize Level of Recognition National (any country) -
2018
Title NÖ Würdigungspreis für besondere wissenschaftliche Lesitungen Type Research prize Level of Recognition Regional (any country)