Glycan structure and biosynthesis in Entamoeba histolytica
- Molecular and biochemical parasitology
- Institute of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna
One PhD position available for the project P31793 “Glycan structure and biosynthesis in Entamoeba histolytica“
Entamoeba histolytica is the amoeba which causes serious dysentery and liver abscess in humans. At the Institute of Specific Prophylaxis and Tropical Medicine we have been studying this protozoan parasite for several decades. The focus of our present project is a major complex surface glycoprotein with the complex name lipopeptidophosphoglycan (LPPG) and its biosynthesis. We plan to hunt for the protein core of the antigen and the enzymes that decorate the core. An LPPG-specific monoclonal antibody will guide us through the preparation of the molecule.
Although the amoebae are covered with glycans, their genome contains only a limited number of enzyme genes for their biosynthesis. We plan to focus on the enzymes that help to make LPPG, to make selected ones in a recombinant form, to perform enzyme assays, and to disrupt their expression in the amoebae. The project is organised as an equal collaboration between the parasitological group in the Medical University of Vienna and the glycobiochemistry group led by Iain Wilson at the University of Natural Resources and Life Sciences (BOKU).
We are looking for a self-motivated and talented scientist with basic skills in molecular biology and at least some experience in biochemistry, who will perform her or his PhD thesis in the Medical University of Vienna. We offer a minimum of 3 ½ years of salary for a 30 h PhD position according to the rules of the FWF.
Applicants should submit a cover letter, a detailed CV, a research summary and contact information for two references to michael.duchene(at)meduniwien.ac.at by February 22.
- Name: Michael Duchene
- Telefon: 01 40160 38210